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	Provedor de dados BJMBR (1) BJM (1) Autor Assumpção,M.C. (1) Benelli,E.M. (1) Bon,Elba Pinto da Silva (1) Carvajal,Elvira (1) Ishida,M.L. (1) Machado,H.B. (1) Pedrosa,F.O. (1) Sotero-Martins,Adriana (1) Souza,E.M. (1) Mais... Palavra-chave Nitrogen regulation (2) Asparaginase II (1) Azospirillum brasilense (1) Genomic integration (1) Green fluorescent protein (1) NtrBC genes (1) NtrYX genes (1) Saccharomyces cerevisiae (1) Two-component regulatory system (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2003 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation BJM Sotero-Martins,Adriana; Bon,Elba Pinto da Silva; Carvajal,Elvira. Production of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II activity, mRNA ASP3, mRNA ASP3-GFP and GFP fluorescence. Nitrogen starvation in cells carrying the chimera ASP3-GFP caused an increase in fluorescence and in the expression of ASP3. We have shown that cells producing the chimera Asp3-GFPp displayed the same response to nitrogen starvation as control cells. We demonstrated that Asp3-GFPp can be used for studying asparaginase II secretion under nitrogen... Tipo: Info:eu-repo/semantics/article Palavras-chave: Asparaginase II; Nitrogen regulation; Saccharomyces cerevisiae; Genomic integration; Green fluorescent protein. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017 Identification and characterization of the two-component NtrY/NtrX regulatory system in Azospirillum brasilense BJMBR Ishida,M.L.; Assumpção,M.C.; Machado,H.B.; Benelli,E.M.; Souza,E.M.; Pedrosa,F.O.. Two Azospirillum brasilense open reading frames (ORFs) exhibited homology with the two-component NtrY/NtrX regulatory system from Azorhizobium caulinodans. These A. brasilense ORFs, located downstream to the nifR3ntrBC operon, were isolated, sequenced and characterized. The present study suggests that ORF1 and ORF2 correspond to the A. brasilense ntrY and ntrX genes, respectively. The amino acid sequences of A. brasilense NtrY and NtrX proteins showed high similarity to sensor/kinase and regulatory proteins, respectively. Analysis of lacZ transcriptional fusions by the ß-galactosidase assay in Escherichia coli ntrC mutants showed that the NtrY/NtrX proteins failed to activate transcription of the nifA promoter of A. brasilense. The ntrYX operon... Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum brasilense; Two-component regulatory system; Nitrogen regulation; NtrYX genes; NtrBC genes. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600004 Registros recuperados: 2 Primeira ... 1 ... Última

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